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iCycler iQ5, iCycler IQ, MJ Opticon, MJ Chromo 4, Rotor-Gene 3000, Rotor-Gene 6000, Rotor-Gene Q or LightCycler 480 do not need ROX reference dye for normalization. 3) Hydrolysis probes such as TaqMan probe cannot be used for melt curve analysis. To take advantage of both probe specificity and melt curve Roto-Gene ® 3000/6000 Rotor-Gene ® Q 5/6 plex Platfom CFX96™ Real-Time System: Downloads. Manual RealStar ® GPR PCR Master 1.0. Product Information Sheet (PIS) pdf. File size: 172.43 KB. Document ID: PIS-8001000-EN-02: Material Safty Data Sheet (MSDS) pdf. File size: 243.46 KB. Document ID: SDS-80010-US-en-01 : Order. Get the product you need. Contact sales. Find your local sales rep or ABI: 7500 (Fast), ViiA 7, QuantStudio 6 and 7 Flex Systems; Stratagene: Mx3000P, Mx3005P and Mx4000; Qiagen/Corbett Rotor-Gene: 3000; Bio-Rad/MJ: Chromo4, Opticon 2 and Opticon 2 Notes: a. 200 nM of primer final concentration is applicable for most cases. The concentration can be adjusted within 0.1~1.0 μM when amplification efficiency Description. SsoAdvanced Universal SYBR Green Supermix is a high-performance real-time PCR supermix based on Bio-Rad's patented* Sso7d fusion protein technology. This supermix is formulated for a wide range of real-time PCR applications and for use with all ROX dependent or independent real-time PCR systems. Diagnostic Sensitivity. 92.86% (CI 95%: 64.17% - 99.63%) Linear Range. 10 10 - 10 2.5 IU/ml with precision of ± 0.5 log. Dynamic Range. 10 10 - 122.594 cp/ml (using manual extraction GeneProof PathogenFree DNA Isolation Kit) 10 10 - 165.237 cp/ml (using automatic extraction croBEE NA16 Nucleic Acid Extraction Kit) Reporting Units. The GeneJET Endo-free Plasmid Maxiprep purification protocol outlined in this manual is optimized for use with bacterial cultures grown in Luria-Bertani (LB) medium to a cell density of approximately 2-3 (OD 600 = 2-3). The use of rich growth media is not recommended. Bacterial culture growth Rotor-Gene™ 3000 Rotor-Gene ™ 6000 Rotor-Gene™ Q Roche Lightcycler®96 Lightcycler®480 Lightcycler®Nano Takara Thermal Cycler Dice® Techne PrimeQ Quantica® Thermo Piko Real™ SensiFAST Selection Table. 5 Table 1 Applications for SensiFAST One‑Step kits Gene expression analysis Microarray validation Viral quantitation Pathogen detection Biomarker discovery and validation Gene Use our combined iScript™ RNase H+reverse transcriptase and antibody hot-start iTaq DNA polymerase kit formulation to complete your SYBR ® Green real-time reactions in one step - PCR efficiency is improved over a wide dynamic range and under various conditions. Advanced formulation for amplifying difficult targets and challenging samples. Qiagen/Corbett Rotor-Gene: 3000; Bio-Rad/MJ: Chromo4, Opticon 2 and Opticon ROX Reference Dye ABI GeneAmp 5700; ABI PRISM 7000, 7700; ABI 7300, 7900HT (Fast); ABI StepOne (Plus) MedChemExpress USA Tel: 609-228-6898 Fax: 609-228-5909 E-mail: sales@MedChemExpress.com SYBR Green qPCR Master Mix MCE USA Tel: 609-228-6898 Email: tech@MedChemExpress.com 1. Prepare PCR reaction mixture To obtain Corbett Rotor-Gene™ 3000 and 6000 Real-Time Rotary Analyzer; Eppendorf Mastercycl