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We recommend using the manual protocol as a guide to develop protocols for automated workstations. MagneGST™ Protein Purification System for Purification.A comprehensive manual. Last date of revision Proceed to protocols for His-tagged protein purification under native conditions (see protocols 4.1 to Proteins are purified using chromatographic purification techniques which separate according to differences in specific properties, as shown in Table 3. Table 3 Setup & Protocol Purpose: Purification of protein samples after the Bugbuster cell lysis. Prepare the buffers needed for protein purification:. The following purification protocol is optimized for purification of His6-tagged GFP. If it is used to purify other proteins, the protocol may have to be