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GSTrap FF Sample: Cytoplasmic extract from E. coli HisTrap HP Sample: Approx. 12 mg histidine-tagged protein in E. coli extract HiTrap Heparin HP Sample: Antithrombin III from bovine plasma HiTrap Desalting Sample: Bovine serum albumin HiTrap Q HP Sample: Human serum, conditions selected to remove IgG fraction in pool 1 HiTrap rProtein A FF ge of pr o te in extracts (M r > 5000). Hi T rap D sal ti n g 1 7-140 8-0 1 0.2 5 to 1.ml t t o 2.0 2 ml P rep ac 5 ml P re packed th w i e S ph adex G-25 Med ium. R u ns by g rav i ty flo w or c entrifug a ti o n Fo r des lt ing, bu ff e e xch an ge, an d c l e an u p of p rot e ins a n h e r l a rg e b io m lecu l e s (M r > 50 00). PD HiTrap IMAC FF 1 ml and 5 ml columns are prepacked with IMAC Sepharose 6 Fast Flow, which consists of 90 µm, highly cross-linked agarose beads with a covalently immobilized chelating group. The medium can easily be charged with Ni 2+, Co , Zn2+, Cu2+, Fe or other metal ions. The high flow-rate properties of IMAC Sepharose 6 Fast Flow make GE Healthcare UK Limited Amersham Place Little Chalfont Buckinghamshire, HP7 9NA UK GE Healthcare Europe, GmbH Munzinger Strasse 5 D-79111 Freiburg Germany GE Healthcare Bio-Sciences Corp. 800 Centennial Avenue, P.O. Box 1327 Piscataway, NJ 08855-1327 USA GE Healthcare Japan Corporation Sanken Bldg., 3-25-1, Hyakunincho Shinjuku-ku, Tokyo 169-0073 Ni Sepharose 6 FF is available in HisTrap FF columns that can be connected in series, as well as larger HisPrep™ FF 16/10 columns when greater capacity is needed. Purification can be performe d directly from unclarified lysates using HisTrap FF crude, His GraviTrap, His SpinTrap, and His MultiTrap columns and 96-well plates. Characteristics of HiTrap DEAE FF, CM FF, Q FF, SP FF and ANX FF columns. 1 Water at room temperature Note: The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium, sample/liquid viscosity and the column tubing used. Supplied Connector kit with HiTrap column Media Instructions 71-7149-00 AN HiTrap ion exchange columns GE Healthcare HiTrap SP HP, 1 ml and 5 ml HiTrap Q HP, 1 ml and 5 ml HiTrap SP HP and HiTrap Q HP are prepacked, ready to use cation and anion exchange columns for method scouting, group separations, sample concentration and sample clean-up of charged biomolecules. company within GE Healthcare that supplies them. A copy of these terms and conditions is available on request. Contact your local GE Healthcare representative for the most current information. GE Healthcare UK Ltd Amersham Place Little Chalfont Buckinghamshire, HP7 9NA, UK GE Healthcare Bio-Sciences Corp 800 Centennial Avenue P.O. Box 1327 HisTrap HP 1 × 1 mL 1 mL 1 column 29051021 Tagged-Package His* - - 29058803 Ni Sepharose 6 Fast Flow ~ 40 mg (his) 6-tagged protein/mL HiTrap column HisTrap FF 5 × 5 mL 5 mL 5 columns 17525501 HisTrap FF crude 5 × 5 mL 5 mL 5 columns 17528601 HisTrap FF 5 × 1 mL 1 mL 5 columns 17531901 HisTrap FF crude 5 × 1 mL 1 mL 5 columns 11000458 Product Type: HiTrap NHS activated HP column: For Use With (Application) Preparative affinity chromato graphy, easy coupling of ligands containing primary Amines instructions i 71-5027-68 Edition AB HiTrap affinity columns HisTrap HP, 1 ml and 5 m