Fluidigm Corporation 2 Tower Place, Suite 2000, South San Francisco, CA 94080 USA +1 650 266 6000 •Toll-free in the US and Canada: 866 359 4354 fluidigm.com For Research Use Only. Not for use in diagnostic procedures. Sample preparation, staining, and analysis • The Maxpar Direct Immune Profiling Assay panel was tested on two frozen human Helios dimensions Width 103 cm (41 in) Height 132 cm (52 in) Depth 87 cm (35 in) Weight 320 kg (705 lb) Chiller size Width 38 cm (15 in) Height 64 cm (25 in) Depth 67 cm (27 in) Weight 81 kg (178 lb) Hyperion Imaging System Channels 135 Mass range 75-209 amu Abundance sensitivity 0.3% for 159Tb Frequency 200 pixels/sec Detection limit The MyDVS portal has a copy of the Helios manual (v6.3.119), which should give you more information. The Helios hardware has some changes as well. The biggest difference is the sample introduction system being pressure-driven from a tube, rather than running through sample loops like on a standard v1 or v2; again, the manual will give more details. This software should not be installed on first-generation CyTOF or CyTOF 2 instrument workstations. Login to the Fluidigm Proteomics Portal by control-clicking this link. Select Data Processing > Software. Download the Software Package for Stand-alone Workstations. Cell-ID 20-Plex Pd Barcoding Kit: User Guide 15 Contains samples introduction settings, mass channels for event detection and monitoring, and CyTOF system settings. A default Settings Template is applied for instrument tuning. For data acquisition, a Settings Template must be applied to each sample in the batch by the user. Fluidigm PRO Services are a comprehensive portfolio of solutions to help you maximize your investment, scale up productivity and empower your laboratory with greater access to professional resources. Hyperion and Hyperion+ Imaging Systems User Guide CyTOF Software v7.0 User Guide MCD Viewer v1.0.560.6 User Guide * The Hyperion Tissue This user guide includes protocols for barcoding samples and for debarcoding multiplexed files. For more information on how palladium barcoding improves data quality and increases workflow efficiencies, see The Benefits of Palladium Barcoding on Data Quality and Workflow Applicati on Note (FLDM-00012). Figure 1. Adjustments to the argon gas flow are required, so read this customer bulletin carefully. In addition, the Helios™ User Guide (PN 400250) has been updated with information on using the WB Injector and Maxpar Cell Acquisition Solution. For Research Use Only. Not for use in diagnostic procedures. See the CyTOF XT User Guide (FLDM-00254). See the CyTOF XT User Guide (FLDM-00254). Specified volume was not properly injected. Incorrect sample loop or nebulizer line is used. Always use Fluidigm recommended replacement parts. • Autosampler Sample Loop, CyTOF® XT. 118763 Mass cytometry, or cytometry by time-of-flight (the basis for Fluidigm® CyTOF® technology), is a system for single-cell detection using antibodies tagged with metal probes. Fluidigm Corporation (2020) Helios, a CyTOF System User Guide (400250) Google Scholar Fluidigm Corporation (2019) Technical note: approach to bivariate analysis of Fluidigm Helios mass cytometer and Hyperion Tissue Imager. Manufacturer: Fluidigm Corporation Model: CyTOF 2 upgraded to Helios mass cytometer, Helios mass cytometer, and Hyperion Tissue Imager Condition: Used. See More Information. Seller InformationAnalytical Technologies Use of this Web site