Here the “Custard apple (Annona Squamosa)” seeds acts one of the natural source of pesticide. The oil is extracted from these seeds by using solvents likeThis paper mainly reveals about extraction of oil from custard apple seeds and the study of its composition and physic-chemical properties. Custard apple seeds In the present investigation, oil is extracted using ethanol as a solvent from custard apple (annona squmocia) seeds and used as bio-pesticide to control pests. In the present studies, oil from custard apple seeds is extracted using hexane or methanol as a solvent and used as bio-pesticide to control the mealy bugs Natural pesticide is those who are made up from natural materials like cow urine, waste leaves, seeds oil etc. It is beneficial to maintain the soil fertility Custard apple seed oil main uses pure pesticides and antales are proud there are medicinal applications of brakes and leaves scontain annonaine an alkaloid