When using the XFluor™ software, the Safire2™can be set up to run the Transcreener® ADP2FP Assay by performing the following steps: 1. Choose "Edit Measurement Parameters" from the XFluor4SafireII dropdown menu. 2. In the General tab, select "Fluorescence Polarization", and "Endpoint". 3. 2 Instructions for Use for INFINITE M1000 PRO No. 30064852 Rev. No. 1.0 2011-09 WARNING Tecan Austria GmbH has taken great care when creating the stored Plate Definition Files (.pdfx) that are supplied with the instrument. manual). : : ' and . . TM: An integrated Gas Control Module (GCM™) offers CO 2 regulation from 0.04 to 10 % (vol.), and O 2 from 0.1 to 21 % (vol.), creating an ideal environment for your cells. CO 2 regulation maintains cell health during long-term kinetic studies, while O 2 control allows you to maintain the correct atmosphere for microaerophilic organisms or mimic Multi-label measurements. Spectral scanning of unknown samples. All major curve fittings are provided - point to point, linear regression, non-linear regression, polynomial, cubic spline, Akima, LogitLog, four- and five-parameter fit. Convenient handling of dilution series and IC50, ICx calculation. Kinetic data analysis. the Tecan Infiniteš M1000 Microplate Reader. Materials ATP/ADP Mixture - 4 mM MgCl 2, 2 mM EGTA, 50 mM HEPES, pH 7.5, 1% DMSO, 0.01% Brij-35, and ATP/ADP (combined to a constant adenine concentration of 10 µM). ADP Detection Mixture - 1X Stop & Detect Buffer B, 4 nM ADP Alexa633 Tracer, and 14.8 µg/mL ADP2 Antibody. Stanford University School of Medicine Safire2TM readers must be appropriately configured for HTRF® readout by setting up the measurement conditions in the "multilabeling" function of Xfluor4 or Magellan software. In particular, these parameters should be entered as below. No special upgrade is required for HTRF® readout, as it is a monochromator-based instrument: Measurement 1 Rapid Detection of DNA Labelling Efficiency. Determination of Labelling Efficiency of cDNA Samples with Fluorophores CY3 and CY5. In this application note we describe the use of a TECAN GENios multifunctional microplate reader for spectrophotometric measurements of CY5. Absorbance scans of CY3 and CY5 have been performed using a TECAN SAFIRE LanthaScreen™ assays can be run on any filter-based instrument capable of time-resolved FRET, such as the Tecan Ultra, BMG PheraStar, Molecular Devices Analyst, or PE Envision. LanthaScreen™ assays have also been performed successfully on the Tecan Safire2 monochromator-based instrument. Contact Invitrogen Technical Services for instrument Tecan safire 2 manual. Tecan safire 2 user manual. Free Pdf Download exe 6 10 2008 1 44 PM 212480 DRV - 2008 01 21 02 23 00 00,019,000 - M CMD Technology, Inc. Every time I tried to type, a. Predictor™ Compatible Microplate Reader Documentation Version No.: 10 Mar 09 Page 2 of 16 Setup Guide on the Tecan Safire2™ Microplate Reader. For a detailed procedure on how to prepare a standard curve, please refer to the appropriate Transcreener®Technical Manual ( bellbrooklabs.com/transcreener_hts_assays.html). Method 1. Dispense 10 μL of each ATP/ADP combination across an entire row of a 384-well plate. 2. Add 10 μL of ADP Detection Mix to those rows. 3. For a detailed procedure on how to prepare a standard curve, please refer to the appropriate Transcreener®Technical Manual ( bellbrooklabs.com/transcreener_hts_assays.html). Method 1. Dispense 10 μL of each ATP/ADP combination acro