10TaqMan®PreAmp Master Mix User Guide Guidelines for preparation of cDNA Guidelines for isolation of high-quality RNA • See Table 4 on page 6 for recommended RNA isolation kits. • (Optional) Use DNase to ensure minimal genomic DNA contamination of the RNA. Guidelines for preparation of cDNA templates 10 TaqMan ® Universal PCR Master Mix User Guide Chapter 1 Product Information 1 Kit contents and storage Kit contents and storage The TaqMan® Universal PCR Master Mix is supplied at 2X concentration and contains: •AmpliTaq Gold® DNA Polymerase, UP (Ultra Pure) •Uracil‐N glycosylase (UNG) •dNTPs with dUTP TaqMan ® Fast Universal PCR Master Mix (2 ) User Guide 13 TaqMan® Fast Universal PCR Master Mix (2 ), No AmpErase® UNG User Guide Perform PCR amplification 4. Save the document. cDNA template guidelines For optimal performance of TaqMan® Fast Universal PCR Master Mix (2 ), No AmpErase® UNG, use 10 ng to 100 ng of cDNA per 20-µL reaction TaqMan ® Fast Universal PCR Master Mix (2 ) User Guide 13 TaqMan® Fast Universal PCR Master Mix (2 ), No AmpErase® UNG User Guide Perform PCR amplification 4. Save the document. cDNA template guidelines For optimal performance of TaqMan® Fast Universal PCR Master Mix (2 ), No AmpErase® UNG, use 10 ng to 100 ng of cDNA per 20-µL reaction Prepare the PCR reaction mix Thaw the preamplified cDNA products on ice. Resuspend the preamplified cDNA products by vortexing, then centrifuge briefly. 1. (Single-tube assays only) Thaw the assays on ice, gently vortex to thoroughly mix, then centrifuge briefly to spin down the contents and eliminate air bubbles. 2.