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Thus, in simple words the spectrophotometer is based on the Beer-Lambert Law which states that the amount of light absorbed is directly proportional to the The human eye responds to radiant energy between approx 390 - 700 nm, called the visible spectrum. c. Modern laboratory instrumentation permits measurements at The basic principle is that every compound absorbs or transmits light over a certain range of frequencies (wavelengths). If we take a measure of this,A spectrophotometer is an instrument that measures the amount of light that can pass through a solution. It is apparent that less light is allowed to pass spectrophotometer,. Principle of additivity of absorbance and simultaneous determination, Spectrophotometry is mainly concerned with the ultraviolet.