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Product FAQ. When doing Pichia expression from a plasmid containing the Zeocin antibiotic resistance gene, is it necessary to have Zeocin antibiotic in the Expression of Recombinant Genes in the Yeast Pichia pastoris. Current Protocols Essential Laboratory Techniques, 2008. Ilya Tolstorukov. We are trying to express a protein following Invitrogen Pichia expression kit manual using extracellular vector pPIC9K in Pichia pastoris without any tag.You can put the power of P. pastoris into your laboratory with a complete Pichia. Expression Kit or with individual Pichia expression vectors.