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Lead generation using pharmacophore mapping and three-dimensional database Assay Guidance Manual. ncgc.nih.gov /guidance/index.html. 19.The Assay Guidance Manual is a guide for scientists working in the early stages of drug development. The manual includes guidelines for selection, This chapter contains guidelines to develop therapeutic hypotheses, target and pathway validation, proof of concept criteria and generalized cost analyses This eBook is a comprehensive, crucial resource for investigators optimizing assays to evaluate collections of molecules with the overall goal of developing process to be quantified using appropriate data analysis procedures. Assay Guidance Manual, Eli Lilly & Company and the National Center for Advancing