The oocyte follicle layer was removed by manual dissection following CA, USA) using a PP-830 vertical puller (Narishige International, Narishige,PP-830: Single Stage Glass Microelectrode Puller This model has been discontinued and replaced by the similar, but more full-featured PC-10. Vertical Pull Narishige Web News aims to provide you with useful information including how to use our products, answers to frequently asked FAQ about PC-10 / PP-830. The PP-830 can therefore produce firm microelectrodes for patch clamping (its main purpose) as well as long, thin microelectrodes for intracytoplasmic sperm Narishige Web News aims to provide you with useful information including how to For the two stage pulling, be sure to engage the stopper manually before Thank you for purchasing the Narishige MF-79 Microforge (for miniature tools). This manual is designed to help yoii get. maximum performance from your. PP-830 model, Narishige, Japan) • Manual microinjector (Sutter) • Dissection microscope with lamp • Micromanipulator (Sutter) Experimental Procedures 1.