Laser scanning confocal microscopy (also called "VK" for the instrument used) is a nondestructive technique which generates 2D and 3D images of a sample surface. Nanometer-scale resolution can be achieved, depending on the focusing lens used for the measurement. Additional Resources. You can select maximum 5 techniques Today's high-resolution laser scanning confocal microscopes (LSCM), also referred to simply as laser scanning microscopes, are powerful high-magnification surface metrology instruments with a vast application range, from simple metal surfaces to high-end electronic components. The RASTMIN setup consists essentially of a custom-made laser-scanning confocal microscope complemented with an xyz stabilization system. A 640 nm pulsed laser (200 ps pulse width at 40 MHz A Laser scanning microscope is a type of optical microscope for imaging a target object using a laser as the light source. Principle of laser confocal microscope There are various types of Laser scanning microscopes depending on the application and detector configuration, so their internal structure and operating principle differ from each other. LSCM Laser Scanning Confocal Microscope Parameter Value Comment Maximum IP Pixel Resolution 1270 x 1000 pixel user adjustable Maximum Field of View 1" CCD equivalent (9.6 x 12.8mm) 800µm diam.at 20X (Example)320µm diam. at 50X (Example) A lens property described as either field curvature or flatness of field is of major importance in many critical measuring and high-resolution imaging applications, such as in semiconductor inspection, although in most biological confocal investigations, it is not a serious limitation. The VK-X3000 has a resolution of 0.01 nm and can scan areas up to 50 x 50 mm (1.97" x 1.97"), allowing for measurement of the overall shape of the target while still maintaining high-resolution for analysis of minute surface features. KEYENCE's new 3D Surface Profiler can handle any target, including those with transparent or mirrored surfaces laser scanning microscope Advantages Greatly increased axial resolution (z axis) Brighter monochromatic illumination from laser Increased resolution in the object plane (x,y Greater sensitivity from detectors with increased Compact storage of images in digital form Ability to process signals and digital images Illustrated in Figure 1 is a Nikon A1R laser confocal microscope scanning unit equipped with a number of advanced features, including a high-resolution galvanometer-based scanner (4,096 x 4,096 pixels; non-resonant) and a high-speed resonant scanner, a continuously variable hexagonal pinhole, spectral imaging capacity, several optical output ports, and a pair of input ports for multiple lasers Fluorescent microscopy not only makes our images look good, it also allows us to gain a better understanding of cells, structures and tissue. With confocal laser scanning microscopy (CLSM) we can find out even more. CLSM combines high-resolution optical imaging with depth selectivity which allows us to do optical sectioning. Generally, we find that scanning at 512 × 512 pixels provides optimal resolution for most live-cell applications. Scanning a larger number of pixels also represents an increase in image acquisition time, which should be minimized with live specimens. The essential feature of the confocal laser scanning microscope (cLSM)