The Yfiler Ⓡ Plus Amplification Kit amplifies 27 Y chromosomal small tandem repeat (STR) markers. The kit has five-fluorescent dye chemistry and the improved PCR buffer system of modern STR kits. We validated the kit for accredited investigations of crime scene samples by a thorough study of kit dynamics and performance. We determined dye-dependent analytical thresholds by receiver operating filexlib. Yfiler® Plus PCR Amplification Kit using a new 6-dye system detects 27 Y-STR loci. This paper describes our validation results of the Yfiler® Plus kit for forensic casework analysis. We examined The AmpFISTR (®) Yfiler (®) Plus (Yfiler Plus) kit appeared to be unimpaired by typical inhibitors such as hematin and humic acid or by large amounts of female components. An upgrade of analyzed markers resulted in increased discrimination capacity that is crucial for forensic trace analysis.
the Yfiler Plus User Guide. For this reason, these artifacts are not further described in the Detailed Artifact Descriptions section of this document. Instead, see the Yfiler Plus kit user guide for more information on these specific artifacts. The data herein is specific to Yfiler Plus kit data run on a 3500 series instrument, and unless
Yfiler™ Plus PCR Amplification Kit - PCR Setup - Extracted DNA Catalog Numbers 4484678 and 4482730 Pub. No. 100030921 Rev. B Note: For safety and biohazard guidelines, see the "Safety" appendix in the Yfiler™ Plus PCR Amplification Kit User Guide (Pub. No. 4485610). Read the Safety Data Sheets (SDSs) and follow the handling instructions.
A total of 1225 unrelated Han males from Henan province were analyzed with the prototype Yfiler® Plus kit (Life Technologies, Thermo Fisher Scientific, Waltham, MA, USA). The calculated gene diversity (GD) values ranged from 0.3855 to 0.9673 for the DYS391 and DYS385a/b loci, respectively. The discriminatory capacity (DC) was 86.94 % with 1065 observed haplotypes using 17 Yfiler loci, by the
NIST
27 Institute of Forensic Medicine, Medical University, Wroclaw, Poland. 28 Illinois State Police, Research & Development Laboratory, Springfield, USA. 29 Department of Forensic Medicine and Criminology, University of Zagreb, Croatia. 30 University Center of Legal Medicine, Lausanne-Geneva, Lausanne, Switzerland.
The Yfiler kit is a powerful tool for the analysis of cases that contain a suspect (s). A significant proportion of DNA cases contain a suspect who has been identified by conventional law enforcement techniques. For these cases, the Yfiler kit is a powerful tool that can be used to include or exclude a suspect (s) associated with an investigation.
Profiling using the Yfiler Plus kit occurred up to 3 years later than profiling in Yfiler due to the later release and implementation of Yfiler Plus at FSSA. Only one extract contained sufficient DNA for it to be amplified at the optimal 400 pg DNA template amount (the optimal input DNA was determined by in-house validation of the Yfiler and

Yfiler plus brugervejledning
Yfiler plus service manual
Yfiler plus handbok
Yfiler plus handboek
Yfiler plus service manual