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Two laboratory tests are used commonly to evaluate coagulation disorders: Prothrombin Time (PT) which measures the integrity of the extrinsic system as well PROCEDURE. A. Manual Method. 1. Bring all reagents, controls and sample to room temperature 15 minutes prior to testing. 2. Pre-warm PT reagent at 37°C for MANUAL PROTIME. The Prothrombin time is a useful screening procedure for deficiencies in factors II, V, VII and X. Deficiencies in factor I, although rare, A prothrombin time test measures how long it takes for your blood plasma to clot. Prothrombin, or factor II, is one of 12 proteins in the clotting process.