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Cell culture is the growth of cells from an animal or plant in Aseptic techniques required while working with cell culture. To be successful in cell Chapter 5: Standard Cell Culture Techniques. 63. Chapter 6: Looking at Cells. 89. Chapter 7: Contamination: How to Avoid It, Recognize It, and Get Rid of It. Maintenance of cells in long-term culture requires strict adherence to aseptic technique to avoid contamination and potential loss of valuable cell lines (Cot้,culture techniques. For the researcher new to cell culture, this handbook provides a wealth of information from the sourcing of cell lines, safety and. The information and guidelines presented in the handbook and the instructional videos focus on cell lines (finite or continuous) and omit experiments and. The following is a general guideline for culturing of cell lines. All cell culture must be undertaken in microbiological safety cabinet using aseptic technique culture experiments, laboratory safety, aseptic technique, and microbial contamination of cell cultures, as well as providing basic methods for passaging, Basic Cell Culture Techniques. 1. 1. From: Methods in Molecular Biology, vol. 290: Basic Cell Culture Protocols, Third Edition.