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PDF - We present a PCR based method to detect Aphanomyces astaci in North American cray- fish. Primers were designed to specifically amplify parts of the internal transcribed spacer (ITS) regions and the 5.8 rRNA gene of A. astaci. A single round and a semi-nested assay were tested for their sensitivity and specificity. Specificity of the PCR assays was tested against several closely related Aphanomyces astaci é uma espécie de oomycota patogénica que provoca uma infeção letal nos lagostins europeus.Na América do Norte, o local de origem destes eucariotas, raramente causa a morte nos lagostins. [1] Embora a doença esteja presente na Europa há cerca de 150 anos, não foram ainda observados lagostins resistentes a esta espécie invasora, que figura na lista das 100 espécies Since 1978 there has been a series of outbreaks of crayfish plague fungus, Aphanomyces astaci in Spain. As a result, most populations of the native freshwater crayfish species, Austropotamobius Request PDF | On Nov 10, 2008, Lage Cerenius and others published Aphanomyces astaci and Crustaceans | Find, read and cite all the research you need on ResearchGate Aphanomyces astaci commonly referred to as crayfish plague is an oomycete or water mould that infects only crayfish species. It is endemic of North America and is carried by North American crayfish species; signal crayfish Pacifastacus leniusculus, Procambarus clarkii and Orconectes limosus.A. astaci was introduced into Europe through imports of North American species of crayfish.